Locked Nucleic Acid Pentamers as Universal PCR Primers for Genomic DNA Amplification
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چکیده
منابع مشابه
Locked Nucleic Acid Pentamers as Universal PCR Primers for Genomic DNA Amplification
BACKGROUND Multiplexing technologies, which allow for simultaneous detection of multiple nucleic acid sequences in a single reaction, can save a lot of time, cost and labor compared to traditional single reaction detection methods. However, the multiplexing method currently used requires precise handiwork and many complicated steps, making a new, simpler technique desirable. Oligonucleotides co...
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The ease and advantages of allelespecific PCR (AS-PCR) for SNP genotyping, along with the difficulty of assay specificity with certain mismatched DNA primers, have been described in prior studies (1–3). More recently, the use of real-time PCR detection formats has been described for one or both alleles of AS-PCR (4–6). These detection enhancement methods do not overcome the inherent difficultie...
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Genomes are becoming heavily annotated with important features. Analysis of these features often employs oligonucleotides that hybridize at defined locations. When the defined location lies in a poor sequence context, traditional design strategies may fail. Locked Nucleic Acid (LNA) can enhance oligonucleotide affinity and specificity. Though LNA has been used in many applications, formal desig...
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The incorporation of locked nucleic acids (LNAs) into oligonucleotide primers has been shown to increase template binding strength and specificity for DNA amplification. Real-time PCR and DNA sequencing have been shown to be significantly enhanced by the use of LNAs. Theoretically, increasing primers' binding strength may also increase the sensitivity of conventional PCR, reducing minimum templ...
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Primers for the amplification and sequencing of DNA fragments from chloroplast genes and non-coding regions are provided to facilitate molecular phylogenetic studies aimed at building a tree-of-life for the Asteraceae. The primers reported here have been extensively tested and some empirical guidelines are included to facilitate their use.
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2008
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0003701